![]() ![]() Although these two methods are popular, some limitations exist. The information of both small metabolites and lipids can be obtained simultaneously without any pretreatment. The latter can detect a few milligrams of intact tissues directly without extraction –. The information of small metabolites can be obtained from spectra of hydrophilic extracts, while fatty acids distribution can be obtained from spectra of lipophilic extracts. The former has been used for HR NMR spectra of extracts from different tissues –. Nowadays, there are two traditional methods for HR NMR spectra of tissues: liquid NMR of tissue extracts and HR magic angle spinning (HR MAS) NMR of ex vivo tissues. Caused by this heterogeneity, line broadening, which often cannot be completely overcome by conventional shimming procedures, may lead to peak overlapping and loss of spectral features. However, muscles, tumors, and connective tissues are generally in semisolid phase, and may be subject to intrinsic variations in magnetic susceptibility over the sample volume. For liquid samples, NMR spectral information can be obtained through conventional liquid high-resolution (HR) NMR spectroscopy, such as chemical shifts and J coupling. In areas of disease diagnosis and drug toxicology, detection results provide much useful information –. Tissue components taken from humans or animals and their percentage variations can be detected by NMR. Nuclear magnetic resonance (NMR) spectroscopy is a non-invasive, non-destructive, pollution-free and safe experimental technique. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This work was supported by the National Natural Science Foundation of China (11074209, 1117423155), ( ), and the Fundamental Research Fund for the Central Universities (2010121008), ( ). Received: JAccepted: DecemPublished: January 17, 2014Ĭopyright: © 2014 Cai et al. PLoS ONE 9(1):Įditor: Patrick van der Wel, University of Pittsburgh School of Medicine, United States of America Citation: Cai H, Chen Y, Cui X, Cai S, Chen Z (2014) High-Resolution 1H NMR Spectroscopy of Fish Muscle, Eggs and Small Whole Fish via Hadamard-Encoded Intermolecular Multiple-Quantum Coherence. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |